ACBP/DBI (E6K8G) XP® Rabbit mAb (#45365) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IHC-P

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P07108

Entrez-Gene Id:

1622

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:250 - 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

ACBP/DBI (E6K8G) XP^® Rabbit mAb recognizes endogenous levels of total ACBP/DBI protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human ACBP/DBI protein.

Background

Acyl-CoA-binding protein (ACBP) binds to long-chain acyl-CoA molecules and regulates lipid metabolism. This protein is also known as diazepam-binding inhibitor (DBI) and binds to the benzodiazepine-binding site of the γ-aminobutyric acid (GABA) type A receptor (GABA_AR), regulating its activity (1). ACBP/DBI is highly expressed in glioblastoma and promotes glioblastoma growth by providing long-chain acyl-CoA molecules to mitochondria for fatty acid oxidation. Reduced ACBP/DBI expression leads to slower proliferation of glioblastoma (2,3). In addition, ACBP/DBI is a regulator of neural progenitor and stem cell proliferation in adult mouse neurogenic niches (4,5). Studies show that ACBP/DBI modulates embryonic neurogenesis of excitatory and inhibitory neurons through GABA signaling (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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