WB, IP
H M R
Endogenous
80-85
Rabbit
#Q15025
10318
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ABIN-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-κB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).
ABIN-1 (Naf1/TNIP1) was identified based on its binding to A20 (6), as well as the HIV protein Nef-1 (7). Overexpression of ABIN-1 inhibits NF-κB activation by a number of stimuli including TNF, IL-1, and LPS. It is widely expressed and can be induced by NF-κB, providing a negative feedback loop of NF-κB signaling. In addition to binding to A20, ABIN-1 can function on other key components of NF-κB signaling including IKKγ/NEMO (8) and NF-κB family members p100 and p105 (9), and can also inhibit ERK signaling (10). Knockout mice of ABIN-1 are embryonic lethal, with fetal liver apoptosis, anemia and hypoplasia (11). ABIN-1 deficient cells are hypersensitive to TNF-mediated apoptosis.
- Verstrepen, L. et al. (2009) Biochem Pharmacol 78, 105-14.
- Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.
- Lee, E.G. et al. (2000) Science 289, 2350-4.
- Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.
- Wertz, I.E. et al. (2004) Nature 430, 694-9.
- Heyninck, K. et al. (1999) J Cell Biol 145, 1471-82.
- Fukushi, M. et al. (1999) FEBS Lett 442, 83-8.
- Mauro, C. et al. (2006) J Biol Chem 281, 18482-8.
- Cohen, S. et al. (2009) Biochem Biophys Res Commun 389, 205-10.
- Zhang, S. et al. (2002) Biochem Biophys Res Commun 297, 17-23.
- Oshima, S. et al. (2009) Nature 457, 906-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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