IF-IC
R
Endogenous
Mouse IgM
Product Information
Product Usage Information
It is strongly recommended that these directions be followed for staining of live, unpermeabilized cells when using this antibody. Dilute primary antibody 1:100 in culture medium containing 0.1% sodium azide*. Aspirate cell culture medium, add diluted antibody and incubate cells for 30 minutes at 37ºC. Aspirate antibody solution, rinse gently with pre-warmed PBS, and fix with methanol-free, 4% formaldehyde diluted in PBS for 15 minutes at 37ºC. Rinse cells with PBS 3 times for 5 minutes each. For best results, detect with a fluorochrome-conjugated anti-mouse IgM secondary antibody (per manufacturer's recommendation, diluted in PBS). Prior to imaging, rinse samples in PBS 3 times for 5 minutes each and mount in an anti-fade reagent (ProLong® Gold Antifade Reagent #9071).
* sodium azide is included to prevent capping.
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with embryonic chicken retinal cells.
Background
A2B5 Mouse mAb recognizes a cell surface ganglioside epitope that has been utilized as a marker for identification of various cell types. A2B5 Mouse mAb has been used to mark specific cell populations such as neuroendocrine cells, thymic epithelial cells (1), and glial precursors that give rise to type II astrocytes and oligodendrocytes (2-4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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