α-Synuclein Antibody (#2628) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IF-F

REACTIVITY:

H M R

SENSITIVITY:

Endogenous

MW (kDa):

SOURCE:

Rabbit

UniProt ID:

#P37840

Entrez-Gene Id:

6622

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:100 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

α-Synuclein Antibody detects endogenous levels of total synuclein protein. Non-specific labeling of cytoplasm in human cells and non-specific labeling of the mucosae in fixed frozen mouse colon may be observed by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse synuclein. Antibodies are purified by peptide affinity chromatography.

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).

Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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