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69319
α-Smooth Muscle Actin (1A4) Mouse mAb (BSA and Azide Free)
一抗
单克隆抗体

α-Smooth Muscle Actin (1A4) Mouse mAb (BSA and Azide Free) #69319

Citations (1)
Filter:
  1. WB
  2. IHC
  3. IF
Western blot analysis of extracts from various tissues using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated) (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, skeletal muscle samples are negative for α-smooth muscle actin. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded normal human colon using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded Syrian hamster ovary using α-Smooth Muscle Actin (1A4) Mouse mAb (IHC Formulated). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse small intestine (left) or skeletal muscle (right) using α-Smooth Muscle Actin (1A4) Mouse mAb (IF Formulated) (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
To Purchase # 69319SF
Cat. # Size Price Inventory
69319SF
100 µg

Supporting Data

REACTIVITY H M R Hm
SENSITIVITY Endogenous
MW (kDa) 42
Source/Isotype Mouse IgG2a

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #56856. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #56856.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

α-Smooth Muscle Actin (1A4) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total α-smooth muscle actin protein.

Species Reactivity:

Human, Mouse, Rat, Hamster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle protein.

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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