| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-Gab1 (Tyr307) Antibody | 3234 | 40 µl | 115 kDa | Rabbit |
| Gab1 Antibody | 3232 | 40 µl | 110 kDa | Rabbit |
| Phospho-Gab2 (Ser159) Antibody | 3884 | 40 µl | 98 kDa | Rabbit |
| Phospho-Gab2 (Tyr452) (C33G1) Rabbit mAb | 3882 | 40 µl | 98 kDa | Rabbit IgG |
| Gab2 (26B6) Rabbit mAb | 3239 | 40 µl | 90 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Gab Antibody Sampler Kit provides an economical means of evaluating levels of Gab1 and Gab2 total protein, as well phosphorylated Gab1 and Gab2 at the specified sites. The kit contains enough reagents to perform four western blot experiments per primary antibody.
Storage
Background
The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).
Gab2, a recently identified docking protein of Gab family, contains a pleckstrin homology domain and potential binding sites for SH2 and SH3 domain-containing proteins. Gab2 has been shown to support growth, differentiation and function in a number of hematopoietic cells (10).
- Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
- Weidner, K.M. et al. (1996) Nature 384, 173-176.
- Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
- Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
- Lehr, S. et al. (1999) Biochemistry 38, 151-159.
- Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
- Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
- Gual, P. et al. (2000) Oncogene 19, 1509-18.
- Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.
- Yi, Q. et al. (2005) Biochem Biophys Res Commun 337, 446-51.
Background References
Trademarks and Patents
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