WB, IP
H M R
Endogenous
23
Rabbit
#P49798
5999
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys33 of human RGS4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Regulators of G protein signaling (RGS) are a family of about 40 proteins that determine the signaling amplitude and duration of G protein-coupled receptor signaling via modulation of the GTPase activity of G proteins (1-3). Each RGS has a distinct structure, expression pattern, and regulation, resulting in preferential interactions with receptors, G proteins, and other signaling proteins, as well as a unique function (4).
RGS4 is a small RGS of 23 kDa that belongs to the R4 subfamily of RGS. It harbors a short N-terminus thought to be critical for its functional specificity towards GPCRs and a RGS homology domain (RH domain) (5). RGS4 shares activity at both Gαq (6) and Gαi/o proteins (7).
RGS4 degradation is regulated by opioid receptor-mediated ubiquitination after stimulation by opioid receptor agonists such as DAMGO (8); and RGS4 modulates the action of morphine and other opiates in vivo (9).
- Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.
- Traynor, J.R. and Neubig, R.R. (2005) Mol Interv 5, 30-41.
- Neubig, R.R. and Siderovski, D.P. (2002) Nat Rev Drug Discov 1, 187-97.
- Terzi, D. et al. (2009) Prog Mol Biol Transl Sci 86, 299-333.
- Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.
- Xu, X. et al. (1999) J Biol Chem 274, 3549-56.
- Berman, D.M. et al. (1996) Cell 86, 445-52.
- Wang, Q. and Traynor, J.R. (2011) J Biol Chem 286, 7854-64.
- Han, M.H. et al. (2010) Biol Psychiatry 67, 761-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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