WB, IP
H M R
Endogenous
12
Rabbit
#Q9CQW9
66141
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln16 of mouse fragilis protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Fragilis (Ifitm3) belongs to the interferon (IFN)-inducible transmembrane protein family (1), which consists of five highly similar transmembrane proteins that are conserved among mammalian species (2). It is thought to be the first gene to mark the onset of germ cell competence during embryonic development in mice (3). Cells expressing high levels of fragilis protein go on to express stella, a protein found exclusively in lineage-restricted germ cells (3). Studies on fragilis family members could help elucidate the mechanisms through which interferons act as signaling molecules during embryonic development (2).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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