WB, IP
H R Mk
Endogenous
95-140
Rabbit
#O94979
22872
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro810 of human Sec31A protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23/24 complex, the Sec13/31 complex, and Sar1. The COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9).
Multiple isoforms of Sec31A protein have been reported (10).
- Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
- Miller, E.A. et al. (2003) Cell 114, 497-509.
- Mossessova, E. et al. (2003) Cell 114, 483-95.
- Barlowe, C. et al. (1994) Cell 77, 895-907.
- Bi, X. et al. (2007) Dev Cell 13, 635-45.
- Shugrue, C.A. et al. (1999) J Cell Sci 112 ( Pt 24), 4547-56.
- Tang, B.L. et al. (2000) J Biol Chem 275, 13597-604.
- Stankewich, M.C. et al. (2006) J Cell Sci 119, 958-69.
- Van Roosbroeck, K. et al. (2011) Blood 117, 4056-64.
- Townley, A.K. et al. (2008) J Cell Sci 121, 3025-34.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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