| Kit Includes | Quantity | Antigen Retrieval / Diluent | Isotype |
|---|---|---|---|
| Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 | 40 µl | EDTA / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 | 40 µl | EDTA / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 | 40 µl | EDTA / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| *SignalStain® Antibody Diluent #8112 | 25 ml | ||
| †SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 | 1 Pack |
*SignalStain® Antibody Diluent is supplied as a working solution and should be stored at 4ºC (packaged separately).
†Control slides should be stored at 4ºC (packaged separately).
#P40763, #P42229, #P42224
6774, 6776, 6772
Product Information
Storage
Specificity / Sensitivity
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1, Tyr705 of mouse Stat3, or Tyr694 of Stat5a.
Product Description
Background
Stat proteins serve as transcription factors in growth and survival pathways stimulated by growth factor and cytokine activation of receptor proteins. Receptor activation promotes tyrosine phosphorylation of Stat proteins, resulting in Stat dimerization and translocation to the nucleus where they regulate expression of numerous proteins that control cell growth, survival, differentiation and pathogen resistance (1). Stat1 is essential in IFN-α and IFN-γ stimulated pathways and is abnormally activated in many tumors (2,3). Both Stat1α (91 kDa) and Stat1β (84 kDa) isoforms are activated by IFN-α but only Stat1α responds to IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Transcription factor Stat3 possesses oncogenic potential and anti-apoptotic activities; a number of human tumors display constitutively activated Stat3 (5,6). Activation of Stat3 follows phosphorylation at Tyr705, resulting in dimerization, nuclear translocation and DNA binding (7). Expression of Stat3α (86 kDa) and Stat3β (79 kDa) isoforms correlates with cell type, ligand and cell maturation stage (8). Phosphorylation of Stat5a at Tyr694 is essential for transcription factor activation (9). Stat5 is activated by interleukins and other cytokines (i.e. CSF1); presence of phosphorylated Stat5 is in some IL-3-treated cells suggests a role in angiogenesis and cell motility (10). Altered Stat5 expression and activity is associated with abnormal cell proliferation and apoptosis in some forms of leukemia (11).
- Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
- Bromberg, J. (2002) J Clin Invest 109, 1139-42.
- Frank, D.A. (1999) Mol Med 5, 432-56.
- Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Darnell, J.E. (1997) Science 277, 1630-5.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
- Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
- Buitenhuis, M. et al. (2004) Int J Biochem Cell Biol 36, 2120-4.
- Baśkiewicz-Masiuk, M. et al. (2003) Cell Prolif 36, 265-78.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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