WB
H M R
Endogenous
27, 28
Rabbit IgG
#O60573
9470
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human 4EHP protein.
Background
Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.
A mammalian cap-binding protein related to eIF4E, 4E Homologous Protein (4EHP), was later identified (6). 4EHP does not interact with eIF4G and, therefore, cannot activate translation (7). Instead, it inhibits the translation of a subset of mRNAs (7).
- Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-47.
- Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-63.
- Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-80.
- Pyronnet, S. et al. (1999) EMBO J. 18, 270-9.
- Raught, B. et al. (2000) EMBO J. 19, 434-44.
- Rom, E. et al. (1998) J Biol Chem 273, 13104-9.
- Zuberek, J. et al. (2007) RNA 13, 691-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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