4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (#18798) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IHC-Bond, IHC-P, IF-F, IF-IC, FC-FP, FC-L

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

24-28, 40-50

Source/Isotype:

Rabbit IgG

UniProt ID:

#P20334

Entrez-Gene Id:

21942

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
IHC Leica Bond	1:100 - 1:400
Immunohistochemistry (Paraffin)	1:200 - 1:800
Immunofluorescence (Frozen)	1:800 - 1:3200
Immunofluorescence (Immunocytochemistry)	1:800 - 1:3200
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400
Flow Cytometry (Live)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #71948.

Specificity / Sensitivity

4-1BB/CD137/TNFRSF9 (E2J5H) XP^® Rabbit mAb recognizes endogenous levels of total 4-1BB/CD137/TNFRSF9 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the extracellular domain of mouse 4-1BB/CD137/TNFRSF9 protein.

Background

TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1,2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

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