Revision 1

#3361

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
50

Source/Isotype:
Rabbit

UniProt ID:
#Q13554, #Q13557, #Q13555, #Q9UQM7

Entrez-Gene Id:
816, 817, 818, 815

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Phospho-CaMKII (Thr286) Antibody detects endogenous levels of CaMKII only when phosphorylated at Thr286.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

CST Logo
Western blot analysis of extracts from Neuro2A cells treated with calcium ionophore (A23187, 2 µM) after a 20-minute preincubation with forskolin (30 μM), using Phospho-CaMKII (Thr286) Antibody (upper) or CaMKII Antibody #3362 (lower).
Western Blotting Image 1: Phospho-CaMKII (Thr286) Antibody
Western blot analysis of extracts from Neuro2A cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-CaMKII (Thr286) Antibody (upper) or CaMKII Antibody #3362 (lower).
Western Blotting Image 2: Phospho-CaMKII (Thr286) Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.