Revision 3
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
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UniProt ID:

#O60674

Entrez-Gene Id:

3717

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb 3776 100 µl H M R 125 Rabbit IgG
Jak2 (D2E12) XP® Rabbit mAb 3230 100 µl H M R 125 Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PhosphoPlus is a registered trademark of Cell Signaling Technology, Inc.
    Select antibodies in this kit are sold under a license from Chemicon International, Inc. relating to U.S. Patent No. 5,658,791.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Revision 3
    #8224

    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet

    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet: Image 1 Expand Image
    使用 Jak2 (D2E12) XP® Rabbit mAb #3230(上图)或 β-Actin (D6A8) Rabbit mAb #8457(下图)对不同细胞系的提取物进行蛋白质印迹法分析。
    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet: Image 2 Expand Image
    使用 Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776(顶图)或总 Jak2 (D2E12) XP® Rabbit mAb #3230(底图)对未处理的或经 GM-CSF 或 IL-3(5 分钟)处理的 UT-7 和 BaF3 细胞的提取物进行蛋白质印迹分析。
    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet: Image 3 Expand Image
    使用 Jak2 (D2E12) XP® Rabbit mAb #3230 和 α-Tubulin (11H10) Rabbit mAb #2125 对经 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 Jak2 siRNA (+) 转染的 K-562 细胞的提取物进行蛋白质印迹分析。Jak2 (D2E12) XP® Rabbit mAb 可确认 Jak2 蛋白表达的沉默,而 α-Tubulin (11H10) Rabbit mAb 则被用于控制 Jak2 siRNA 的上样和特异性。
    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet: Image 4 Expand Image
    使用 Jak2 (D2E12) XP® Rabbit mAb 对 K-562、THP-1、TF-1 和 BaF3 细胞系提取物进行蛋白质印迹分析。
    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet: Image 5 Expand Image
    使用 Jak2 (D2E12) XP® Rabbit mAb 对石蜡包埋的人肺癌进行免疫组织化学分析。
    PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet: Image 6 Expand Image
    在对照肽(左图)或 Jak2 Blocking Peptide #1039(右图)存在的情况下,使用 Jak2 (D2E12) XP® Rabbit mA 对石蜡包埋的人肺癌进行免疫组织化学分析。