Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
ESET (C1C12) Rabbit mAb 2196 40 µl 180 kDa Rabbit IgG
Fibrillarin (C13C3) Rabbit mAb 2639 40 µl 37 kDa Rabbit IgG
Histone H2A.Z Antibody 2718 40 µl 14 kDa Rabbit 
Histone H3 (D1H2) XP® Rabbit mAb 4499 40 µl 17 kDa Rabbit IgG
Lamin A/C (4C11) Mouse mAb 4777 40 µl 74 (Lamin A), 63 (Lamin C) kDa Mouse IgG2a
LSD1 (C69G12) Rabbit mAb 2184 40 µl 110 kDa Rabbit IgG
NUP98 (C39A3) Rabbit mAb 2598 40 µl 98 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl Horse 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit provides an economical means to evaluate relevant nuclear proteins. This kit contains enough primary antibody to perform at least four western blots per primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit contains a variety of antibodies directed against established nuclear proteins (1). Histone H3 and histone H2A.Z are histone family members and components of nucleosomes, the primary building block of chromatin made up of DNA wound around eight core histone proteins. The amino-terminal tails of core histones undergo various post-translational modifications and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (2). ESET histone methyltransferase (3) and LSD1 histone demethylase (4) are both regulators of histone methylation and are chromatin-associated. Both NUP98 (5) and lamins (6) are located within the nuclear envelope (also known as the nuclear membrane). NUP98 is a component of the nuclear pore complex. Lamin A and lamin C are fibrous proteins contributing to nuclear structural and transcriptional regulation. Finally, fibrillarin (7) is located in fibrillar regions and Cajal bodies of nucleoli, where it functions to regulate RNA transcription and pre-rRNA processing.

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
  3. Yang, L. et al. (2002) Oncogene 21, 148-52.
  4. Shi, Y. et al. (2004) Cell 119, 941-53.
  5. Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
  6. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  7. Tollervey, D. et al. (1993) Cell 72, 443-57.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。
    Revision 1
    #12784

    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit

    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 1 Expand Image
    对使用 Fibrillarin (C13C3) Rabbit mAb（左图，绿色）、DyLight™ 554 Phalloidin #13054（右图，红色）和 DAPI #8961（右图，蓝色）标记的固定冷冻的小鼠肝脏进行共聚焦免疫荧光分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 2 Expand Image
    对使用 Fibrillarin (C13C3) Rabbit mAb（左图，绿色）、DyLight™ 554 Phalloidin #13054（右图，红色）和 DAPI #8961（右图，蓝色）标记的固定冷冻的小鼠肾脏进行共聚焦免疫荧光分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 3 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb（实线）或浓度匹配的 Mouse (G3A1) mAb IgG1 Isotype Control #5415（虚线）对 Daudi 细胞（蓝色）和 MJ 细胞（绿色）进行流式细胞分析。Anti-mouse IgG (H+L)，F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 作为二抗。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 4 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb #4777 对 Hela 细胞的裂解物 (0.1 mg/mL) 进行 Simple Western™ 分析。虚拟泳道式图像（左图）显示一抗稀释比例在 1:10 和 1:50 时的靶标条带（如图所示）。对应的电泳图（右图）为一抗稀释比例在 1:10（蓝线）和 1:50（绿线）时沿毛细血管内分子量的化学发光结果。在还原条件下，使用 12-230 kDa 分离模块在 ProteinSimple（BioTechne 品牌）的 Jess™ Simple Western 仪器上进行该实验。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 5 Expand Image
    使用 LSD1 (C69G12) Rabbit mAb 对不同细胞类型的细胞裂解物进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 6 Expand Image
    使用 ESET (C1C12) Rabbit mAb 对 MCF7 和 293细胞的细胞裂解液进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 7 Expand Image
    使用 NUP98 (C39A3) Rabbit mAb，对不同细胞类型的细胞提取物进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 8 Expand Image
    使用核仁纤维蛋白 (C13C3) 抗体对不同细胞类型提取物进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 9 Expand Image
    使用 Histone H2A.Z Antibody #2718（上）和 Histone H2A Antibody II #2578（下），对加入 10 ng 的重组 H2A (rH2A) 和 H2A.Z (rH2A.Z) 蛋白的 HeLa、NIH/3T3、H-4-II-E、COS 细胞裂解物，进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 10 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb 对不同细胞系提取物进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 11 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 12 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb #4777（上图）或 α-actinin (D6F6) XP® Rabbit mAb #6487（下图）对对照 HeLa 细胞（泳道 1）或 LMNA 编码基因出现明显框内截短突变的 HeLa 细胞（泳道 2）的提取物进行蛋白印迹分析。突变 HeLa 细胞中 LMNA 分子量的变化与框内缺失一致。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 13 Expand Image
    一抗与靶标蛋白结合之后，与偶联 HRP 的二抗形成复合体。添加 LumiGLO®，在酶催化分解期间发光。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 14 Expand Image
    一抗与靶标蛋白结合之后，与偶联 HRP 的二抗形成复合体。添加 LumiGLO*，它可在酶催化的分解期间发光。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 15 Expand Image
    使用 LSD1 (C69G12) Rabbit mAb 对石蜡包埋的人乳腺癌进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 16 Expand Image
    使用 ESET (C1C12) Rabbit mAb（绿色）对 MCF-7 细胞进行共聚焦免疫荧光分析。肌动蛋白纤丝用 Alexa Fluor®555 phalloidin（红色）进行标记。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 17 Expand Image
    使用 NUP98 (C39A3) Rabbit mAb（绿色），对 Caki-1 细胞进行共聚焦免疫荧光分析。肌动蛋白纤丝用 Alexa Fluor®555 phalloidin（红色）进行标记。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 18 Expand Image
    使用 Fibrillarin (C13C3) Rabbit mAb（绿色）对 HeLa 细胞进行共聚焦免疫荧光分析。肌动蛋白纤丝用 Alexa Fluor®555 phalloidin（红色）进行标记。蓝色伪彩 = DRAQ5®（DNA 荧光染料）。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 19 Expand Image
    使用 Histone H2A.Z Antibody（绿色），对 HeLa 细胞进行共聚焦免疫荧光分析。肌动蛋白纤丝用 Alexa Fluor®555 phalloidin（红色）进行标记。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 20 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb 对石蜡包埋的 4T1 同基因乳腺肿瘤组织进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 21 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb 对不同细胞系提取物进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 22 Expand Image
    使用 LSD1 (C69G12) Rabbit mAb 对石蜡包埋的人肺癌进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 23 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb（绿色）和 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116（红色）对 HeLa 细胞进行共聚焦免疫荧光分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 24 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb 对石蜡包埋的 LL/2 同基因肿瘤组织进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 25 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb 对未经处理或经 Cycloheximide 处理（CHX，10 μg/ml，过夜）后再经 TNF-α #8902处理（20 ng/ml，4 小时）的 THP-1 细胞提取物进行蛋白质印迹分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 26 Expand Image
    使用 LSD1 (C69G12) Rabbit mAb 对石蜡包埋的人胃癌进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 27 Expand Image
    以浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（虚线）作为对照，使用 Histone H3 (D1H2) XP® Rabbit mAb（实线）对 Hela 细胞进行流式细胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 28 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb 对石蜡包埋的小鼠脑组织进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 29 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb 对石蜡包埋的人乳腺癌细胞进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 30 Expand Image
    使用 Histone H3 (D1H2) XP® Rabbit mAb 对石蜡包埋的恒河猴肝组织进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 31 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb 对石蜡包埋的人结肠癌细胞进行免疫组织化学分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 32 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb （绿色）和 MAP2 Antibody #4542 （红色）对正常大鼠脑细胞进行免疫荧光分析。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 33 Expand Image
    使用 LSD1 (C69G12) Rabbit mAb（绿色）对 HeLa 细胞进行共聚焦免疫荧光分析。肌动蛋白纤丝用 Alexa Fluor®555 phalloidin（红色）进行标记。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 34 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb （绿色）对 HeLa 细胞进行共聚焦免疫荧光分析。使用 DY-554 phalloidin（红色）标记肌动蛋白纤丝。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 35 Expand Image
    使用 Lamin A/C (4C11) Mouse mAb（实线）或浓度匹配的 Mouse (G3A1) mAb IgG Isotype Control #5415（虚线），对 HeLa 细胞（绿色）进行流式细胞分析。Anti-mouse IgG (H+L)，F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 作为二抗。
    Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit: Image 36 Expand Image
    使用 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003，对 U-937 细胞中提取的交联染色质，在加入 LSD1 (C69G12) Rabbit mAb 或Normal Rabbit IgG #2729 后，进行染色质免疫沉淀。使用 SimpleChIP® Human CD11b Promoter Primers #14271、人 SPI-1 启动子引物和 SimpleChIP® Human α Satellite Repeat Primers #4486 通过实时 PCR 对富集 DNA 进行定量分析。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。