#P35236
5778
Product Information
Storage
Store at -80° C.
Keep on ice during use.
Avoid repeated freeze-thaw cycles.
Source / Purification
The GST-phosphatase fusion protein was produced by expressing recombinant human PTPN7 (Met1-Pro360) (GenBank Accession No. NM_002832) with an amino-terminal GST tag in E. coli. The protein was purified by one-step affinity chromatography using glutathione-agarose.
Product Description
| MW (kDa) | 66000 |
Background
Hematopoietic protein tyrosine phosphatase (HePTP, PTPN7) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells (1). The protein is made up of a short, amino-terminal extension that contains a conserved kinase interaction motif (KIM) and a carboxy-terminal protein tyrosine phosphatase domain (1,2). The presence of the kinase interaction motif allows PTPN7 to interact with MAPK family kinases, such as Erk1/2 and p38 MAP kinases (2,3). Reciprocal modification occurs between PTPN7 and Erk2, as the kinase phosphorylates PTPN7 at Thr45 and Ser72 while the phosphatase dephosphorylates Erk2 at Tyr185 (2). Down regulation of MAPK signaling follows removal of specific phospho-tyrosine resides from these MAPK kinases (3,4). Modification of MAPK family kinases by PTPN7 can regulate both kinase activity and localization (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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