41, 45
#P19419
2002
Product Information
Product Usage Information
Elk 1 Fusion Protein, at a concentration of 2 µg/20 µl reaction, can be phosphorylated using 50 units of p42 MAP kinase (Erk2) in an in vitro kinase assay with 1X Kinase Buffer #9802 and 200 µM ATP #9804. After a 30-minute assay at 30ºC, phosphorylation can be detected by Western blot with Phospho-Elk-1 (Ser383) Antibody #9181.
Storage
Source / Purification
Cloned from a human cDNA library (7) and overexpressed in E. coli.d in E. coli.
Product Description
| MW (kDa) | 41, 45 |
| Activity | Elk 1 Fusion Protein at a concentration of 2 µg/20 µ| reaction was phosphorylated using p42 MAP kinase (Erk1) in an in vitro kinase assay with 1X Kinase Buffer #9802 and 200 µM ATP #9804. After a 30-minute assay at 30°, phosphorylation was detected by Western blot with Phospho-Elk-1 (Ser383) Antibody #9181. |
| Molecular Formula | Apparent Molecular Weight: 41 kDa |
Background
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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