| Product Includes | Product # | Quantity | Color | Storage Temp |
|---|---|---|---|---|
| Caveolin-1 Rabbit mAb Coated Microwells | 79739 | 96 tests |
|
+4C |
| Caveolin-1 Mouse Detection mAb | 12553 | 1 ea |
|
+4C |
| Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) | 13304 | 1 ea |
|
+4C |
| Detection Antibody Diluent | 13339 | 11 ml |
|
+4C |
| HRP Diluent | 13515 | 11 ml |
|
+4C |
| TMB Substrate | 7004 | 11 ml |
|
+4C |
| STOP Solution | 7002 | 11 ml |
|
+4C |
| Sealing Tape | 54503 | 2 ea |
|
+4C |
| ELISA Wash Buffer (20X) | 9801 | 25 ml |
|
+4C |
| ELISA Sample Diluent | 11083 | 25 ml |
|
+4C |
| Cell Lysis Buffer (10X) | 9803 | 15 ml |
|
-20C |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The PathScan® Total Caveolin-1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total caveolin-1 protein. A caveolin-1 rabbit mAb has been coated onto the microwells. After incubation with cell lysates, caveolin-1 protein is captured by the coated antibody. Following extensive washing, Caveolin-1 Mouse Detection mAb is added to detect the captured caveolin-1 protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of total caveolin-1 protein.
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).
- Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
- Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
- Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
- Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
- Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
- Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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