Revision 7

#29443Store at +4C

1 个试剂盒

(96 assays)

Species Cross Reactivity

H M R

UniProt ID:

#P10636-6, #P10636-7, #P10636-8

Entrez-Gene Id:

#4137

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Color Storage Temp
Tau Rabbit mAb Coated Microwells 47827 96 tests +4C
Tau 4R Rabbit Detection mAb 66222 1 ea Red (Lyophilized) +4C
HRP Diluent 13515 5.5 ml Red +4C
TMB Substrate 7004 11 ml +4C
STOP Solution 7002 11 ml +4C
Sealing Tape 54503 2 ea +4C
ELISA Wash Buffer (20X) 9801 25 ml +4C
Cell Lysis Buffer (10X) 9803 15 ml -20C

*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Description

The rapid protocol (RP) PathScan® RP Tau 4R Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Tau 4R protein (Tau 4R iso6/iso7/iso8) in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Tau 4R protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Tau 4R protein. Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

The PathScan® RP Tau 4R Sandwich ELISA Kit detects endogenous levels of Tau 4R isoforms 6, 7, and 8, and does not cross-react with Tau 3R isoforms 2, 4, or 5, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Alternative splicing of exon 10 results in the expression of two groups of tau; three-repeat and four-repeat tau. Isoforms 2, 4, and 5 express three microtubule binding repeat domains (Tau 3R) while isoforms 6, 7, and 8 express four microtubule binding repeat domains (Tau 4R) (4). The localization of Tau 3R and Tau 4R in cells can be expressed differently in mild or pathological conditions, including Tau 3R in Pick's disease (PiD) and corticobasal degeneration (CBD), while in Alzheimer's disease equally expresses both Tau 3R and Tau 4R (5,6). The repeat-dependent tau has a different pattern of phosphorylation in different diseases, and also has the ability and patterns of aggregation (7-9).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Šimić, G. et al. (2016) Biomolecules 6, 6.
  5. Tuerde, D. et al. (2018) J Biol Chem 293, 1781-1793.
  6. Liu, C. and Götz, J. (2013) PLoS One 8, e84849.
  7. Weismiller, H.A. et al. (2018) J Biol Chem 293, 17336-17348.
  8. Goedert, M. et al. (2018) Cold Spring Harb Symp Quant Biol 83, 163-171.
  9. Kraus, A. et al. (2019) Acta Neuropathol 137, 585-598.

Background References

    Cross-Reactivity Key

    H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Revision 7
    #29443

    PathScan® RP Tau 4R Sandwich ELISA Kit

    PathScan® RP Tau 4R Sandwich ELISA Kit: Image 1 Expand Image
    图 1. PathScan® RP Tau 4R Sandwich ELISA Kit #29443 检出 Tau 4R 同工型 6、7 和 8,但不检出 Tau 3R 同工型 2、4 或 5。左图中显示使用 PathScan® RP Tau 4R Sandwich ELISA Kit #29443 对未经转染或经编码所指定 Tau 3R 同工型和 Tau 4R 同工型的表的表达构建体瞬时转染的293T 细胞的细胞提取物 (0.01 mg/ml) 时 450 nm 处的吸光度。使用 Tau 4R Antibody(上图)和 Tau Antibody(下图)检测到的相应蛋白质印迹如下图所示。
    PathScan® RP Tau 4R Sandwich ELISA Kit: Image 2 Expand Image
    图 2. 显示了小鼠脑提取物的裂解物蛋白浓度与使用 PathScan® RP Tau 4R Sandwich ELISA Kit #29443 时 450 nm 处吸光度之间的关系。