Revision 1

#7281Store at 4C and -20C

 

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Species Cross Reactivity

H

UniProt ID：

#P42229, #P51692

Entrez-Gene Id：

#6776, #6777

Cell Signaling Technology

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For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Volume Cap Color Storage Temp

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Description

CST's PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Kit #7113. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Phospho-Stat5 (Tyr694) Mouse Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Stat5 Rabbit Detection Antibody and an HRP-conjugated Anti-Rabbit IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Stat5 phosphorylated at Tyr694.
*Antibodies in this kit are custom formulations specific to the kit.

Reagents not supplied

Phosphate Buffered Saline (PBS-20X) #9808 Phosphate Buffered Saline with Tween-20 (PBST-20X) #9809 Cell Lysis Buffer (10X) #9803 TMB Substrate #7004 STOP Solution #7002 Blocking Buffer: 1X PBS/0.05% Tween-20, 1% BSA 96 Well Microplates** Microplate Reader ** Antibody Pairs have been validated on Corning® 96 Well Clear Polystyrene High Bind Stripwell™ Microplates (#2592) and Corning® 96 Well EIA/RIA Easy Wash™ Clear Flat Bottom Polystyrene High Bind Microplates (#3369).

Background

Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

  1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
  4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
  5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
  6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

Background References

    Cross-Reactivity Key

    H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。
    Revision 1
    #7281

    PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Antibody Pair

    PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Antibody Pair: Image 1 Expand Image
    The relationship between the protein concentration of the lysate from untreated and EGF-treated HeLa cells and the absorbance at 450 nm using PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Antibody Pair #7281 is shown. HeLa cells were treated with EGF (100 ng/mL) for 40 minutes at 37ºC and then lysed.
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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。