| Product Includes | Product # | Quantity | Color |
|---|---|---|---|
| FastScan™ ELISA Microwell Strip Plate, 96 Well | 53257 | 96 tests | |
| CD79B Rabbit Capture mAb | 70493 | 1 ea | Green (Lyophilized) |
| CD79B Rabbit HRP-linked mAb | 49739 | 1 ea | Red (Lyophilized) |
| FastScan™ ELISA Capture Antibody Diluent | 16076 | 3 ml | Green |
| FastScan™ ELISA HRP Antibody Diluent | 28120 | 3 ml | |
| TMB Substrate | 7004 | 11 ml | |
| STOP Solution | 7002 | 11 ml | |
| Sealing Tape | 54503 | 1 ea | |
| ELISA Wash Buffer (20X) | 9801 | 25 ml | |
| FastScan™ ELISA Cell Extraction Buffer (5X) | 69905 | 10 ml | |
| FastScan™ ELISA Cell Extraction Enhancer Solution (50X) | 25243 | 1 ml | |
| FastScan™ ELISA Kit #25553 Positive Control Type 1 | 29485 | 1 ea |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The FastScan™ Total CD79B ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CD79B. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with CD79B in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of CD79B.
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
The B cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12).
- Mason, D.Y. et al. (1995) Blood 86, 1453-9.
- Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
- Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
- Reth, M. and Wienands, J. (1997) Annu Rev Immunol 15, 453-79.
- Kurosaki, T. and Kurosaki, M. (1997) J Biol Chem 272, 15595-8.
- Dal Porto, J.M. et al. (2004) Mol Immunol 41, 599-613.
- Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
- van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
- Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
- Hashimoto, S. et al. (1995) Mol Immunol 32, 651-9.
- Dornan, D. et al. (2009) Blood 114, 2721-9.
- Pfeifer, M. et al. (2015) Leukemia 29, 1578-86.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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