FastScan™ Phospho-TBK1/NAK (Ser172) ELISA Kit (#46948) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Color
FastScan^™ ELISA Microwell Strip Plate, 96 Well	53257	96 tests
Phospho-TBK1/NAK (Ser172) Rabbit Capture mAb	59777	1 ea	Green (Lyophilized)
TBK1/NAK Rabbit HRP-linked mAb	74257	1 ea	Red (Lyophilized)
FastScan^™ ELISA Capture Antibody Diluent	16076	3 ml	Green
FastScan^™ ELISA HRP Antibody Diluent	28120	3 ml
TMB Substrate	7004	11 ml
STOP Solution	7002	11 ml
Sealing Tape	54503	1 ea
ELISA Wash Buffer (20X)	9801	25 ml
FastScan^™ ELISA Cell Extraction Buffer (5X)	69905	10 ml
FastScan^™ ELISA Cell Extraction Enhancer Solution (50X)	25243	1 ml
FastScan^™ ELISA Kit #46948 Positive Control Type 2	89559	1 ea

*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Description

The FastScan^™ Phospho-TBK1/NAK (Ser172) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of TBK1/NAK when phosphorylated at Ser172. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-TBK1/NAK (Ser172) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-TBK1/NAK (Ser172).

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

The FastScan^™ Phospho-TBK1/NAK (Ser172) ELISA Kit detects endogenous levels of TBK1/NAK when phosphorylated at Ser172, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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