Product Includes | Product # | Quantity | Color |
---|---|---|---|
FastScan™ ELISA Microwell Strip Plate, 96 Well | 53257 | 96 tests | |
Stat6 Rabbit Capture mAb | 48519 | 1 ea | Green (Lyophilized) |
Phospho-Stat6 (Tyr641) Rabbit HRP-linked mAb | 63604 | 1 ea | Red (Lyophilized) |
FastScan™ ELISA Capture Antibody Diluent | 16076 | 3 ml | Green |
FastScan™ ELISA HRP Antibody Diluent | 28120 | 3 ml | |
TMB Substrate | 7004 | 11 ml | |
STOP Solution | 7002 | 11 ml | |
Sealing Tape | 54503 | 1 ea | |
ELISA Wash Buffer (20X) | 9801 | 25 ml | |
FastScan™ ELISA Cell Extraction Buffer (5X) | 69905 | 10 ml | |
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) | 25243 | 1 ml | |
FastScan™ ELISA Kit #58967 Positive Control Type 1 | 61781 | 1 ea |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The FastScan™ Phospho-Stat6 (Tyr641) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat6 when phosphorylated at Tyr641. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-Stat6 (Tyr641) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-Stat6 (Tyr641).
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
- Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
- Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
- Hou, J. et al. (1994) Science 265, 1701-1706.
- Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
- Takeda, K. et al. (1996) Nature 380, 627-630.
- Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
- Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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