FastScan™ Phospho-4E-BP1 (Thr37/46) ELISA Kit (#49118) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Color
FastScan^™ ELISA Microwell Strip Plate, 96 Well	53257	96 tests
4E-BP1 Rabbit Capture mAb	15523	1 ea	Green (Lyophilized)
Phospho-4E-BP1 (Thr37/46) Rabbit HRP-linked mAb	31583	1 ea	Red (Lyophilized)
FastScan^™ ELISA Capture Antibody Diluent	16076	3 ml	Green
FastScan^™ ELISA HRP Antibody Diluent	28120	3 ml
TMB Substrate	7004	11 ml
STOP Solution	7002	11 ml
Sealing Tape	54503	1 ea
ELISA Wash Buffer (20X)	9801	25 ml
FastScan^™ ELISA Cell Extraction Buffer (5X)	69905	10 ml
FastScan^™ ELISA Cell Extraction Enhancer Solution (50X)	25243	1 ml
FastScan^™ ELISA Kit #49118 Positive Control Type 1	75635	1 ea

*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Description

The FastScan^™ Phospho-4E-BP1 (Thr37/46) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of 4E-BP1 when phosphorylated at Thr37/46. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-4E-BP1 (Thr37/46) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-4E-BP1 (Thr37/46).

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

The FastScan^™ Phospho-4E-BP1 (Thr37/46) ELISA Kit detects endogenous levels of 4E-BP1 when phosphorylated at Thr37/46, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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