Revision 3

#82818Store at +4C

1 个试剂盒

(96 assays)

Species Cross Reactivity

H

UniProt ID：

#P68431

Entrez-Gene Id：

#8350

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Color
FastScan™ ELISA Microwell Strip Plate, 96 Well 53257 96 tests
Histone H3 Rabbit Capture mAb 10606 1 ea Green (Lyophilized)
Citrullinated Histone H3 (Arg17) Rabbit HRP-linked mAb 28967 1 ea Red (Lyophilized)
FastScan™ ELISA Capture Antibody Diluent 16076 3 ml Green
FastScan™ ELISA HRP Antibody Diluent 28120 3 ml
TMB Substrate 7004 11 ml
STOP Solution 7002 11 ml
Sealing Tape 54503 1 ea
ELISA Wash Buffer (20X) 9801 25 ml
FastScan™ ELISA Cell Extraction Buffer (5X) 69905 10 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) 25243 1 ml
FastScan™ ELISA Kit #82818 Positive Control Type 2 96313 1 ea

*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Description

The FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H3 when citrullinated at Arg17. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with citrullinated histone H3 (Arg17) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of citrullinated histone H3 (Arg17).

*Antibodies in kit are custom formulations specific to kit.

IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.

Specificity/Sensitivity

The FastScan™ Citrullinated Histone H3 (Arg17) ELISA Kit detects endogenous levels of histone H3 when citrullinated at Arg17, as shown in Figure 1. This kit may also recognize histone H3 citrullinated at residue Arg26. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone post-translational modifications, such as methylation, acetylation, ubiquitination, and citrullination, influence chromatin accessibility and regulate gene expression (1). Histone citrullination is carried out by protein arginine deiminases (PADs), which convert positively charged arginine residues into neutral citrulline (2). Histone citrullination affects the binding of histone reader proteins to chromatin, and it is associated with both transcriptional activation and repression (3-5). Histone citrullination is also a major marker for NETosis, which is a programmed cell death mechanism carried out by neutrophils (6). De-regulation of NETosis is associated with multiple autoimmune diseases, including rheumatoid arthritis (RA) and lupus (7,8). Histone hypercitrullination via PAD4 may also play a role in the progression of various cancers, including multiple myeloma (MM), acute myeloid leukemia (AML), lung cancer, and hepatocellular carcinoma (HCC) (9-12).

  1. Shanmugam, M.K. et al. (2018) Oncotarget 9, 11414-11426.
  2. Fuhrmann, J. and Thompson, P.R. (2016) ACS Chem Biol 11, 654-68.
  3. Wang, Y. et al. (2004) Science 306, 279-83.
  4. Cuthbert, G.L. et al. (2004) Cell 118, 545-53.
  5. Zhang, X. et al. (2012) Proc Natl Acad Sci U S A 109, 13331-6.
  6. Hamam, H.J. and Palaniyar, N. (2019) Biomolecules 9, 369. doi: 10.3390/biom9080369.
  7. Corsiero, E. et al. (2016) Front Immunol 7, 485.
  8. Hakkim, A. et al. (2010) Proc Natl Acad Sci U S A 107, 9813-8.
  9. McNee, G. et al. (2017) Leukemia 31, 373-381.
  10. Song, G. et al. (2016) Oncotarget 7, 3144-57.
  11. Tanikawa, C. et al. (2012) Nat Commun 3, 676.
  12. Lu, M. et al. (2020) J Med Virol 92, 1221-1230.

Background References

    Cross-Reactivity Key

    H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。