Product Includes | Product # | Quantity | Color | Storage Temp |
---|---|---|---|---|
MUC1 Mouse mAb Coated Microwells | 51119 | 96 tests |
|
+4C |
MUC1 Rabbit Detection mAb | 83369 | 1 ea |
|
+4C |
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) | 13272 | 1 ea |
|
+4C |
Detection Antibody Diluent | 13339 | 11 ml |
|
+4C |
HRP Diluent | 13515 | 11 ml |
|
+4C |
TMB Substrate | 7004 | 11 ml |
|
+4C |
STOP Solution | 7002 | 11 ml |
|
+4C |
Sealing Tape | 54503 | 2 ea |
|
+4C |
ELISA Wash Buffer (20X) | 9801 | 25 ml |
|
+4C |
ELISA Sample Diluent | 11083 | 25 ml |
|
+4C |
Cell Lysis Buffer (10X) | 9803 | 15 ml |
|
-20C |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The PathScan® Total MUC1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of MUC1 protein. A MUC1 mouse mAb has been coated onto the microwells. After incubation with cell lysates, the MUC1 proteins are captured by the coated antibody. Following extensive washing, a MUC1 rabbit detection mAb is added to detect captured MUC proteins. Anti-rabbit, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of MUC1 protein.
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6) and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8).
- Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231.
- Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64.
- Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24.
- Li, Y. et al. (2001) J Biol Chem 276, 6061-4.
- Ren, J. et al. (2002) J Biol Chem 277, 17616-22.
- Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47.
- Raina, D. et al. (2004) J Biol Chem 279, 20607-12.
- Wei, X. et al. (2005) Cancer Cell 7, 167-78.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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