The rapid protocol (RP) PathScan^® RP Total Stat6 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat6 protien in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Stat6 protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Stat6 protien. Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

PathScan^® RP Total Stat6 Sandwich ELISA Kit detects endogenous levels of Stat6 protein in human cells, as shown in Figure 1, and mouse cells, as shown in Figure 2. The kit sensitive is shown in Figure 1 and 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).