| Product Includes | Product # | Quantity | Color | Storage Temp |
|---|---|---|---|---|
| B7-H4 Rabbit mAb Coated Microwells | 26894 | 96 tests |
|
+4C |
| B7-H4 Rabbit Detection mAb | 66040 | 1 ea |
|
+4C |
| HRP Diluent | 13515 | 5.5 ml |
|
+4C |
| TMB Substrate | 7004 | 11 ml |
|
+4C |
| STOP Solution | 7002 | 11 ml |
|
+4C |
| Sealing Tape | 54503 | 2 ea |
|
+4C |
| ELISA Wash Buffer (20X) | 9801 | 25 ml |
|
+4C |
| Cell Lysis Buffer (10X) | 9803 | 15 ml |
|
-20C |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The rapid protocol (RP) PathScan® RP Total B7-H4 Sandwich ELISA Kit Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of B7-H4 protein in a reduced assay time of 1.5 hours. Incubation of cell lysate and detection antibody on the coated microwell plate forms a sandwich with B7-H4 protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of B7-H4 protein. Learn more about all of your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to the kit.
Specificity/Sensitivity
Background
B7 homolog 4 (B7-H4, VTCN1) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses (1-3). B7-H4 protein contains two extracellular Ig-like V-type domains, a transmembrane domain, and a short, two amino acid intracellular domain (3). The B7-H4 protein is shown to inhibit T cell activation, proliferation, and cytokine production (1,4,5). Although B7-H4 mRNA is widely expressed, B7-H4 protein is restricted to antigen presenting cells and B cells (1). The B7-H4 protein is also found in several tumor types, including ovarian cancer and breast cancer (6). Research studies indicate that B7-H4 protein is present on the surface of ovarian tumor cells, and that targeted inhibition of B7-H4 using recombinant antibodies restores T cell activation pathways. These studies suggest some potential therapeutic value in blocking B7-H4 function and restoring T cell function in cancer patients (7,8).
- Sica, G.L. et al. (2003) Immunity 18, 849-61.
- Prasad, D.V. et al. (2003) Immunity 18, 863-73.
- Zang, X. et al. (2003) Proc Natl Acad Sci U S A 100, 10388-92.
- He, C. et al. (2011) Clin Dev Immunol 2011, 695834.
- Yi, K.H. and Chen, L. (2009) Immunol Rev 229, 145-51.
- Salceda, S. et al. (2005) Exp Cell Res 306, 128-41.
- Dangaj, D. and Scholler, N. (2013) Oncoimmunology 2, e25913.
- Dangaj, D. et al. (2013) Cancer Res 73, 4820-9.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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