9
#P48061
6387
Background
SDF1, also known as CXCL12 and PBSF, is an 8 kDa member of the CXC family of chemokines (1). SDF1 is highly conserved across species and the human protein differs from mouse by only one conserved amino acid substitution (2). SDF1α and SDF1β are splice variants which are identical with regard to amino acid sequence except for an additional 4 amino acids on the carboxy terminus of SDF1β (3). Both SDF1 isoforms binds to the G protein-coupled receptor, CXCR4 (4). SDF1β is a potent lymphocyte chemoattractant (5). SDF1/CXCR4 signaling is required for B cell lymphopoiesis and myelopoiesis (6,7). While CXCR4 signaling plays a key role in breast cancer metastasis, increased SDF1 expression in breast cancer may be a prognostic indicator of overall survival (8).
- Zlotnik, A. and Yoshie, O. (2000) Immunity 12, 121-7.
- Shirozu, M. et al. (1995) Genomics 28, 495-500.
- Tashiro, K. et al. (1993) Science 261, 600-3.
- Roland, J. et al. (2003) Blood 101, 399-406.
- Bleul, C.C. et al. (1996) J Exp Med 184, 1101-9.
- Ma, Q. et al. (1998) Proc Natl Acad Sci U S A 95, 9448-53.
- Nagasawa, T. et al. (1996) Nature 382, 635-8.
- Mirisola, V. et al. (2009) Eur J Cancer 45, 2579-87.
Endotoxin
Less than 0.01 ng endotoxin/1 μg hSDF1β.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hSDF1β. All lots are greater than 98% pure.
Source / Purification
Recombinant human SDF1β (hSDF1β) Lys22-Met93 (Accession #NP_000600) was produced in E. coli at Cell Signaling Technology.
Bioactivity
The activity of hSDF1β was determined using an NK-92 cell migration assay. The ED50 of each lot is between 0.1-0.3 µg/ml.
Background
SDF1, also known as CXCL12 and PBSF, is an 8 kDa member of the CXC family of chemokines (1). SDF1 is highly conserved across species and the human protein differs from mouse by only one conserved amino acid substitution (2). SDF1α and SDF1β are splice variants which are identical with regard to amino acid sequence except for an additional 4 amino acids on the carboxy terminus of SDF1β (3). Both SDF1 isoforms binds to the G protein-coupled receptor, CXCR4 (4). SDF1β is a potent lymphocyte chemoattractant (5). SDF1/CXCR4 signaling is required for B cell lymphopoiesis and myelopoiesis (6,7). While CXCR4 signaling plays a key role in breast cancer metastasis, increased SDF1 expression in breast cancer may be a prognostic indicator of overall survival (8).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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