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#P34130
4909
Background
NT-4 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-3 (1). NT-4 is expressed in a number of cell types and tissues, including neuronal cells, normal breast epithelial cells, melanocytes, activated T cells, and granulocytes (1-5). NT-4 is required for the development of peripheral sensory neurons (6,7). NT-4 may be important for the development of long term memory (8). Increased NT-4 expression in melanoma cells promotes cell proliferation and migration (5). NT-4 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-4 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkB. While all neurotrophins bind to the p75NTR receptor, NT-4 preferentially binds to the TrkB receptor (1).
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- Laurenzi, M.A. et al. (1998) J Leukoc Biol 64, 228-34.
- Truzzi, F. et al. (2008) J Invest Dermatol 128, 2031-40.
- Conover, J.C. et al. (1995) Nature 375, 235-8.
- Liu, X. et al. (1995) Nature 375, 238-41.
- Xie, C.W. et al. (2000) Proc Natl Acad Sci U S A 97, 8116-21.
Endotoxin
Less than 0.01 ng endotoxin/1 μg hNT-4.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNT-4. All lots are greater than 98% pure.
Source / Purification
Recombinant human Neurotrophin-4 (hNT-4) Gly81-Ala210 (Accession #NP_006170) was produced in E.coli at Cell Signaling Technology.
Bioactivity
The activity of hNT-4 was assessed by quatification of phospho-TrkB in TrkB transfected NIH/3T3 cells. The observed ED50 values are between 1-4 ng/ml.
Background
NT-4 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-3 (1). NT-4 is expressed in a number of cell types and tissues, including neuronal cells, normal breast epithelial cells, melanocytes, activated T cells, and granulocytes (1-5). NT-4 is required for the development of peripheral sensory neurons (6,7). NT-4 may be important for the development of long term memory (8). Increased NT-4 expression in melanoma cells promotes cell proliferation and migration (5). NT-4 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-4 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkB. While all neurotrophins bind to the p75NTR receptor, NT-4 preferentially binds to the TrkB receptor (1).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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