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#P41159
3952
Background
Leptin is a 16 kDa adipocyte-derived hormone that relays the status of the body’s energy reserves to the hypothalamus, resulting in the suppression of appetite and modulation of energy expenditure (1,2). Leptin functions as a pleiotropic cytokine with multiple roles in immune function and reproduction (1-4). There are at least five isoforms of the leptin receptor, but only OB-Rb is signaling competent (1,2). OB-Rb is expressed on a number of cells, including T cells, B cells, keratinocytes, and others (1-6). Leptin/OB-Rb interactions induce Jak2, PI3K, Erk1/2, and Stat3 activation (1,2). OB-Rb is often highly expressed in many tumor types, including breast, pancreatic, and Tumor Initiating Stem Cells (TISCS), providing a potential mechanistic link for the tumor promoting effects of obesity (2,7).
- Faggioni, R. et al. (2001) FASEB J 15, 2565-71.
- Park, J. et al. (2011) Endocr Rev 32, 550-70.
- Mancuso, P. et al. (2011) J Immunol 186, 1081-90.
- Hausman, G.J. and Barb, C.R. (2010) Endocr Dev 19, 31-44.
- Kanda, N. and Watanabe, S. (2008) Endocrinology 149, 5189-98.
- Tanaka, M. et al. (2011) J Neurosci 31, 8373-80.
- Feldman, D.E. et al. (2012) Proc Natl Acad Sci U S A 109, 829-34.
Endotoxin
Less than 0.01 ng endotoxin/1 μg hLeptin.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLeptin. All lots are greater than 98% pure.
Source / Purification
Recombinant human Leptin (hLeptin) Val22-Cys167 (Accession #NP_000221) was produced in E.coli at Cell Signaling Technology.
Bioactivity
The bioactivity of recombinant hLeptin was determined by its ability to induce TNF-α production from MCF7 cells. The minimum concentration used to obtain this effect was 50 µg/ml.
Background
Leptin is a 16 kDa adipocyte-derived hormone that relays the status of the body’s energy reserves to the hypothalamus, resulting in the suppression of appetite and modulation of energy expenditure (1,2). Leptin functions as a pleiotropic cytokine with multiple roles in immune function and reproduction (1-4). There are at least five isoforms of the leptin receptor, but only OB-Rb is signaling competent (1,2). OB-Rb is expressed on a number of cells, including T cells, B cells, keratinocytes, and others (1-6). Leptin/OB-Rb interactions induce Jak2, PI3K, Erk1/2, and Stat3 activation (1,2). OB-Rb is often highly expressed in many tumor types, including breast, pancreatic, and Tumor Initiating Stem Cells (TISCS), providing a potential mechanistic link for the tumor promoting effects of obesity (2,7).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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