19.3
#P20809
3589
Background
Interleukin-11 (IL-11) was initially cloned as a mediator of plasmacytoma cell proliferation and was later found to exhibit a wide variety of biological effects in neural cells as well as in the hematopoietic and immune systems (1). IL-11 belongs to the interleukin-6 (IL-6)-type subfamily of long-chain helical cytokines, including IL-6, ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), oncostatin M, and cardiotrophin-1, which all share the glycoprotein gp130 as a signal transducing receptor component. IL-11 acts on cells expressing gp130 and the IL-11 receptor (IL-11R) α subunit. Both receptor subunits belong to the family of class I cytokine receptors. The complex of IL-11 and IL-11R triggers the activation of gp130 most likely by enforcing gp130 homodimerization (2). As a consequence of gp130 activation, several cytoplasmic signal transduction cascades are initiated from the Janus kinase (Jak)/signal transducer and activator of transcription (Stat) pathway, which has attracted considerable attention. Initiation of the Jak/Stat pathway in response to IL-11 requires Jak1 and leads predominantly to the activation of Stat3 (3,4).
Endotoxin
Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-11.
Purity
A greater than or equal to 95% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human IL-11 was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant hIL-11 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 10 ng/ml.
Background
Interleukin-11 (IL-11) was initially cloned as a mediator of plasmacytoma cell proliferation and was later found to exhibit a wide variety of biological effects in neural cells as well as in the hematopoietic and immune systems (1). IL-11 belongs to the interleukin-6 (IL-6)-type subfamily of long-chain helical cytokines, including IL-6, ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), oncostatin M, and cardiotrophin-1, which all share the glycoprotein gp130 as a signal transducing receptor component. IL-11 acts on cells expressing gp130 and the IL-11 receptor (IL-11R) α subunit. Both receptor subunits belong to the family of class I cytokine receptors. The complex of IL-11 and IL-11R triggers the activation of gp130 most likely by enforcing gp130 homodimerization (2). As a consequence of gp130 activation, several cytoplasmic signal transduction cascades are initiated from the Janus kinase (Jak)/signal transducer and activator of transcription (Stat) pathway, which has attracted considerable attention. Initiation of the Jak/Stat pathway in response to IL-11 requires Jak1 and leads predominantly to the activation of Stat3 (3,4).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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