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#P09038
2247
Background
FGF basic (FGF2) is produced by epithelial, tumor and other cell types (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).
- Dvorak, P. and Hampl, A. (2005) Folia Histochem Cytobiol 43, 203-8.
- Ornitz, D.M. and Itoh, N. (2001) Genome Biol 2, REVIEWS3005.
- Shi, Y. et al. (2008) Crit Rev Oncol Hematol 65, 43-53.
- Fontijn, D. et al. (2006) Br J Cancer 94, 1627-36.
- Marek, L. et al. (2009) Mol Pharmacol 75, 196-207.
- Acevedo, V.D. et al. (2009) Cell Cycle 8, 580-8.
Endotoxin
Less than or equal to 1 EU / 1 μg hFGF-basic
Purity
A greater than 95% purity was determined by SDS-PAGE.
Source / Purification
Human FGF-basic Recombinant Protein was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of Human FGF-basic Recombinant Protein was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05 - 1.0 ng/ml.
Background
FGF basic (FGF2) is produced by epithelial, tumor and other cell types (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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