15-25
#Q9ES17
60505
Background
IL-21 is a pleiotropic type I cytokine that is produced by CD4+ T cells, Th17 cells and NKT cells (1,2). The IL-21 receptor is a heterodimer that consists of an IL-21R and the common γ chain, γc (1). IL-21/IL-21R signaling involves activation of JAKs1/3, STAT3 and, to a lesser extent, STAT5 and STAT1 (1,2). IL-21 promotes B cell proliferation, survival and differentiation into plasma cells (3,4). IL-21 activates the cytolytic functions of CD8+ T cells and NK cells, and has been shown in mouse models to play a key role in immunity to chronic viral infections and promote anti-tumor immunity (5-7).
- Leonard, W.J. et al. (2008) J Leukoc Biol 84, 348-56.
- Spolski, R. and Leonard, W.J. (2008) Curr Opin Immunol 20, 295-301.
- Parrish-Novak, J. et al. (2000) Nature 408, 57-63.
- Parrish-Novak, J. et al. (2002) J Leukoc Biol 72, 856-63.
- Pan, X.C. et al. (2013) Oncol Lett 5, 90-96.
- Fröhlich, A. et al. (2009) Science 324, 1576-80.
- Yi, J.S. et al. (2009) Science 324, 1572-6.
Endotoxin
Less than 0.01 ng endotoxin/1 μg mHis6IL-21.
Purity
>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-21. All lots are greater than 95% pure.
Source / Purification
Recombinant mouse His6IL-21 (mHis6IL-21) Pro25-Ser146 (Accession #NP_068554.1) was expressed in human 293 cells at Cell Signaling Technology.
Bioactivity
The bioactivity of recombinant mHis6IL-21 was determined in a B9 cell proliferation assay. The ED50 of each lot is between and 20-80 pg/ml.
Background
IL-21 is a pleiotropic type I cytokine that is produced by CD4+ T cells, Th17 cells and NKT cells (1,2). The IL-21 receptor is a heterodimer that consists of an IL-21R and the common γ chain, γc (1). IL-21/IL-21R signaling involves activation of JAKs1/3, STAT3 and, to a lesser extent, STAT5 and STAT1 (1,2). IL-21 promotes B cell proliferation, survival and differentiation into plasma cells (3,4). IL-21 activates the cytolytic functions of CD8+ T cells and NK cells, and has been shown in mouse models to play a key role in immunity to chronic viral infections and promote anti-tumor immunity (5-7).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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