22-35
#P20826
17311
Background
SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).
Endotoxin
Less than 0.01 ng endotoxin/1 μg mSCF.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mSCF. All lots are greater than 98% pure.
Source / Purification
Recombinant mouse SCF (mSCF) Lys26-Ala189 (Accession #NP_038626) was expressed in human 293 cells at Cell Signaling Technology.
Bioactivity
The bioactivity of recombinant mSCF was determined in an M-07e cell proliferation assay. The ED50 of each lot is between 2-8 ng/ml.
Background
SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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