Mouse His6 Tumor Necrosis Factor-alpha (mHis6TNF-alpha) #4698
Inquiry Info. # 4698
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Product Information
Formulation
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mHis6TNF-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Storage
Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 20 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6TNF-α. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg mHis6TNF-α. |
| Activity | The bioactivity of recombinant mHis6TNF-α was determined in a L-929 cell viability assay. The ED50 of each lot is between 10-40 pg/ml. |
| Molecular Formula | Recombinant N-terminally His6-tagged mTNF-α has a calculated MW of 20203. DTT-reduced and non-reduced protein migrate as 20 kDa polypeptides. |
Source / Purification
Recombinant mouseHis6 TNF-α (mHis6TNF-α) Leu80-Leu235 (Accession #NP_038721) was expressed in human 293 cells at Cell Signaling Technology.
Background
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK and NF-κB) promotes the survival of cells, while TNF-α mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
Alternate Names
his tag; tnf-a; tnfa; TNFSF2
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For Research Use Only. Not for Use in Diagnostic Procedures.
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