Mouse GM-CSF Recombinant Protein

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is produced by activated T cells, NK cells, and macrophages (1,2). Target cells include granulocytes, monocyte precursors, and subsets of differentiated myeloid cells (1,3,4). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in the hematopoietic differentiation of dendritic cells, and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (5). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5, and PI3K/Akt pathways (1).

Endotoxin levels are less than or equal to 1 EU / 1 μg mGM-CSF.

A greater than or equal to 95% purity was determined by SDS-PAGE.

Recombinant mouse GM-CSF was expressed in E. coli and is supplied in a lyophilized form.

The bioactivity of recombinant mGM-CSF was determined in an FDC-P1 cell proliferation assay. The ED₅₀ of each lot is less than or equal to 0.05 ng/ml.