15.1
#P05112
3565
Background
Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
- Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
- Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
- Kopf, M. et al. (1993) Nature 362, 245-8.
- Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
- Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
- Hou, J. et al. (1994) Science 265, 1701-6.
Endotoxin
Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-4.
Purity
A greater than or equal to 95% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human IL-4 was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant hIL-4 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 0.25 ng/mL.
Background
Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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