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#P39905
2668
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson’s disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer’s Disease (AD), and has been associated with cancer progression (6-9).
- Fielder, G.C. et al. (2018) Neoplasia 20, 99-117.
- Choi-Lundberg, D.L. et al. (1997) Science 275, 838-41.
- Sariola, H. and Saarma, M. (2003) J Cell Sci 116, 3855-62.
- Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
- Konishi, Y. et al. (2014) J Neurosci 34, 13127-38.
- Cintrón-Colón, A.F. et al. (2020) Cell Tissue Res 382, 47-56.
- Sharif, M. et al. (2021) Neurol Sci 42, 2865-2872.
- Choi, Y.D. et al. (2020) Int J Mol Sci 21, 3586. doi: 10.3390/ijms21103586.
- Yu, Z. et al. (2022) Int J Oncol 61, 109. doi: 10.3892/ijo.2022.5399.
Endotoxin
Endotoxin levels are less than or equal to 1 EU / 1 μg hGDNF.
Purity
A greater than or equal to 95% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human GDNF was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant hGDNF was determined in a C6 cell proliferation assay. The ED50 of each lot is less than or equal to 3 μg/ml.
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson’s disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer’s Disease (AD), and has been associated with cancer progression (6-9).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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