DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag)

Use as directed in the respective SimpleChIP^® Chromatin IP kit, the CUT&RUN Assay kit, and the CUT&Tag Assay kit protocols. Please note that if following the SimpleChIP^® Chromatin protocol or the CUT&Tag protocol, there will be excess DNA Binding Buffer (#10007) to accommodate the CUT&RUN protocol.

Store at room temperature. Components are stable for 12 months upon receipt when stored properly.

This product is offered to enable DNA purification using spin columns with our CUT&RUN Assay Kit (#86652).

This product also is offered to conveniently provide additional buffer reagents when using our SimpleChIP^® (#9002, #9003) and SimpleChIP^® Plus (#9004, #9005) Enzymatic Chromatin IP Kits, SimpleChIP^® Sonication Chromatin IP Kit (#56383), and CUT&Tag Assay Kit (#77552). These kits provide all the reagents required for performing the respective assays, however there are instances where extra buffers are desired.

The DNA purification columns combine the convenience of spin-column technology with the selective binding properties of a uniquely designed silica membrane that allows for efficient recovery of DNA and removal of protein contaminants without the need for phenol/chloroform extractions and ethanol precipitations. After DNA purification, the enrichment of particular DNA sequences can be analyzed by a variety of methods.