TUNEL Assay Kit (Fluorescence, 488 nm) (#25879) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-P, IF-IC, FC-FP

Product Information

Storage

All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Specificity / Sensitivity

Tissue processing or cell degradation may result in labeling of nuclei in non-apoptotic cells.

Product Description

TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) is a method by which a fluorophore conjugated nucleotide is enzymatically linked to the 3′ end of fragmented DNA. Since DNA fragmentation is a hallmark of apoptosis, the TUNEL assay has become a well established method to monitor apoptosis in situ. The TUNEL Assay Kit (Fluorescence, 488 nm) provides you with the buffers, enzyme, and dUTP fluorophore conjugate necessary to complete the TUNEL reaction in fixed cells or tissue. This kit is intended for use with fluorescence microscopy and/or flow cytometry.

Background

Apoptosis is a regulated cellular suicide mechanism characterized by nuclear condensation, cell shrinkage, membrane blebbing, and DNA fragmentation (1). During late stages of apoptosis, DNA is fragmented by an endonuclease that cleaves the chromatin into nucleosomal units which can be visualized on gels as DNA laddering (2). DNA fragmentation also serves as a basis for monitoring apoptosis in situ using the TUNEL assay (3). In this assay, terminal deoxynucleotidyl transferase enzymatically incorporates a fluorophore conjugated nucleotide to the 3' end of fragmented DNA. Apoptosis can be monitored by an increase in TUNEL staining within intact cells.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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