Serine/Threonine Kinase Substrate Screening Kit (#7400) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Storage

Antiboidies are supplied in 50 mM Tris-HCl (pH 7.4), 150 mM NaCl (TBS), 0.05% Tween-20, 1.0 µg/ml BSA. Store at 4°C. Do not aliquot the antibodies.
Peptides are supplied at 12.5 µM in 1X kinase buffer (25 mM Tris (pH 7.5), 5 mM beta-glycerolphosphate, 2 mM DTT, 0.1 mM Na3V04, 5 mM MgCl2). Store peptieds at -20°C.

Specificity / Sensitivity

10 to 1000+ fold signal-to-noise ratio of phospho- versus nonphospho-peptides measured by DELFIA^® assay.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are corresponding to mice immunized with synthetic phosphopeptides .

Product Description

The Serine/Threonine Kinase Substrate Screening Kit provides 87 biotinylated nonphospho-peptides as kinase substrates. Also included are biotinylated phospho and nonphospho-peptides of identical sequence as positive and negative controls respectively. Potential substrate phosphorylation is detected by the corresponding phospho-specific antibodies.
The paired phospho-specific antibody and peptide substrate can be used for high throughput peptide based kinase screens (e.g. FP, TRF, AlphaScreen).

Background

In order to expedite the isolation of substrates for novel kinases, Cell Signaling Technology has developed a kinase/substrate development kit. Numerous serine/threonine kinases have become pharmaceutically important, including GSK, Akt and p38. With the cloning of hundreds of uncharacterized serine/threonine kinases, drug discovery researchers are seeking to quickly find substrates for high throughput screening programs.
With a total of 87 different peptide/phospho-specific antibody pairs on the array, a very diverse group of substrates is represented. Signal-to-noise ratios have been optimized and range from 10 to over 1000. The phospho-specific antibody and peptide substrate pair can be used for subsequent high throughput peptide based kinase screens (e.g., FP, TRF, AlphaScreen).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专