Revision 3
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

FC-FP

:

:

:

:

UniProt ID:

#P00722

Entrez-Gene Id:

945006

Product Information

Storage

Store lyophilized or in solution at -20ºC. In lyophilized form, the chemicals are stable for 24 months. Once in solution, use Bafilomycin A1 within three months, and use SA-β-Gal Fluorescent Substrate within one month.

Product Description

The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry) allows the quantitative measure of senescence-associated β-galactosidase activity in living cells via flow cytometry using a cell permeable fluorogenic substrate, which becomes fluorescent upon hydrolysis by β-galactosidase. The fluorescent molecule is optimally excited at 490 nm (blue laser) and peak emission occurs at 514 nm (usually FL1). Each kit contains sufficient quantities of reagents to perform up to 100 assays.

Background

Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent β-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.

  1. Goldstein, S. (1990) Science 249, 1129-33.
  2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci., USA 85, 9086-90.
  3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-7.
  4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
  5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-51.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 3
#35302

Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry)

Flow Cytometry Image 1: Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry) Expand Image
以未标记的 MCF6 细胞(虚线)作为比较,使用 SA-β-Gal Fluorescent Substrate(实线)对未经过处理(蓝色)或经过 Etoposide #2200处理(12.5 μM、24 小时,绿色)并经过 3 天复原的 MCF7 细胞进行流式细胞分析。