LDH 细胞毒性测定试剂盒

All components in this kit are stable for at least 12 months when stored at the recommended temperature.

The LDH Cytotoxicity Assay Kit can be used for quantitatively measuring cytotoxicity in response to chemical compounds as well as assaying cell-mediated cytotoxicity using a coupled two-step, colorimetric reaction. In the first step, lactate dehydrogenase (LDH) catalyzes the reduction of NAD+ to NADH and H+ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H+ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan, which absorbs strongly at 490-520 nm. The amount of formazan produced is proportional to the amount of LDH released into the culture medium as a result of cytotoxicity.

In a prototypical cytotoxicity assay, target cells are cultured with a cytotoxic chemical agent or a cytotoxic cell (e.g., NK cells) to induce target cell death and LDH release. The LDH-containing supernatants are transferred to wells of a new assay plate and mixed with the LDH Reaction Solution. After an incubation of 30 minutes at room temperature, the absorbance at 490 nm (A490) is read using a plate reader. Cells treated with cytotoxic agents or cytotoxic cells will release an amount of LDH that falls between the maximum release control level and the spontaneous release control level.