Revision 3

#70976Store at 4C and -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
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Product Information

Storage

All components in this kit are stable for 12 months when stored at the recommended temperature.

Specificity / Sensitivity

The Human Anti-CD3/CD28 T Cell Activation Kit is used for the stimulation of human T cells following the soluble stimulation protocol.

Product Description

The Human Anti-CD3/CD28 T Cell Activation Kit provides the necessary antibodies to induce human T cell activation and proliferation using the soluble stimulation protocol.

Background

T cells are activated by signaling through ITAM (immunoreceptor tyrosine-based activation motif)-containing CD3 signaling chains that associate with the T cell receptor (1). Co-stimulation through CD28 on T cells provides an additional signal required for effective T cell activation (2). Signaling through both CD3 and CD28 can be initiated using the agonist antibodies Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) and Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free), respectively. If the agonist antibodies are used in the soluble stimulation protocol, cross-linking of their Fc domains using Goat Anti-Mouse Kappa Light Chain, F(ab')2 Antibody (Low Endotoxin, Azide-free) is required for optimal stimulation.

  1. Pitcher, L.A. and van Oers, N.S. (2003) Trends Immunol 24, 554-60.
  2. June, C.H. et al. (1990) Immunol Today 11, 211-6.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 3
#70976

Human Anti-CD3/CD28 T Cell Activation Kit

Human Anti-CD3/CD28 T Cell Activation Kit: Image 1 Expand Image
使用 Phospho-SLP-76 (Ser376) (D7S1K) Rabbit mAb #92711(上图)、SLP-76 (E4N7E) Rabbit mAb #25361(中图)或 β-Actin (D6A8) Rabbit mAb #8457(下图)对未经处理(泳道 1)或经过 Human Anti-CD3/CD28 T Cell Activation Kit #70976(30 分钟)(泳道 2)处理的 Jurkat 细胞进行蛋白质印迹分析。