HTScan® Profiling Kit (Tyrosine Kinase Set I) (#7405) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

UniProt ID:

#P54753, #P00533, #P22607, #P08581, #P09619, #P08069, #P36888, #P35968

Entrez-Gene Id:

2049, 1956, 2261, 4233, 5159, 3480, 2322, 3791

Product Information

Storage

Antibodies are supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Do not aliquot the antibodies. Store at -20°C. Peptides are supplied at 6 µM in 0.001% DMSO or a DMSO and carbonate buffer solution (15 mM Na2C03, 35 mM NaHC03, 0.05% NaN3). Store at -20°C. Enzymes are supplied in 50 mM Tris-HCL (pH 8.0), 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione and 20% glycerol. Store at -80°C.
Keep enzmes on ice during use.
Avoid repeated freeze-thaw cycles.

Specificity / Sensitivity

The purity of each RTK was characterized using SDS-PAGE, and assay conditions for phosphorylation of the included substrates were optimalized using DELFIA^® (for details, visit www.cellsignal.com/drug2.AS) (DELFIA^® is a registered trademark of PerkinElmer, Inc.).

Source / Purification

Each GST-Kinase fusion protein in this kit was produced using a baculovirus-expressed cDNA fragment with the target human tyrosine kinase domain amino-terminally fused to a GST-HIS6-Thrombin cleavage site. Proteins were then purified by one-step affinity chromatography using glutathione-agarose.
The detection antibody, Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411, was corresponding to mice immunized with phospho-tyrosine-containing peptides .

Product Description

The kit provides a means of characterizing the effect of a compound or panel of compounds on the included active human receptor tyrosine kinases.
The tyrosine kinases in the kit are supplied as GST fusion proteins. Biotinylated substrate peptides validated for each kinase are supplied at 6µM, and a phosphotyrosine monoclonal antibody for detection of the phosphorylated form of the substrate peptide is also included in the kit. The kit enables rapid characterization of the effect of a single compound at multiple concentrations or a panel of compounds toward the following RTKs: PDGFR-beta, EGFR, FGFR-3, VEGFR-2, FLT3, IGF1R, MET and EPHB3, each representing an RTK subgroup.

Background

Tyrosine kinases have been validated as targets for therapeutic intervention in many disease areas. The human genome codes for nearly one hundred tyrosine kinases, which can be categorized into receptor tyrosine kinases (RTKs) and cytosolic tyrosine kinase. RTKs are currently a major focus for therapeutic drug target screening in the pharmaceutical industry. HTScan¬Profiling Kit (Tyrosine Kinase Set 1) from Cell Signaling Technology (CST) is an antibody-based kinase activity assay kit.

Each kinase is paired with a validated biotynilated substrate peptide, provided in 96-well format. Optimal assay conditions have been identified for each substrate peptide using a phosphotyrosine-specific antibody to achieve signal-to noise ratios over 25.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专