#P11362
2260
Product Information
Storage
5 mM DTT, 15 mM reduced glutathione and 20%
glycerol. Store at –80°C.
Keep enzymes on ice during use.
Avoid repeated freeze-thaw cycles.
Source / Purification
The GST-Kinase fusion protein was produced using a baculovirus expression system with a construct expressing a fragment of human FGFR-1 (Gly400-Arg820) with an amino-terminal GST tag. The protein was purified by one-step affinity chromatography using glutathione-agarose.
Product Description
Molecular Formula | Peptide substrate, Biotin-Pyk2 (Tyr402): 2,166 Daltons. GST-FGFR-1 Kinase: 78 kDa. |
Background
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors-FGF receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated and the receptor tyrosine kinase activated (2), resulting in further activation of various downstream signaling molecules and lysosomal degradation. FGFR has peptide substrate specificity similar to other receptor tyrosine kinase members, prefering acidic residues at the -1 to -4 positions and large hydrophobic amino acids at positions +1 and +3 (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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