#P41240
1445
Product Information
Source / Purification
The GST-Kinase fusion protein was produced using a baculovirus expression system with a construct expressing Met1-Leu450 of the human Csk (GenBank Accession No.NM_004383) with an amino-terminal GST tag. The protein was then purified by one-step affinity chromatography using glutathione-agarose.
Product Description
| Molecular Formula | Peptide substrate, Biotin-peptide: 2,290 Daltons, GST-Csk Kinase: 80 kDa. |
Background
Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFKs) by phosphorylation of the SFK carboxy-terminal tyrosine (1,2). Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK (3). This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk (4).
Csk consists of an SH2, an SH3, and a kinase domain. There is evidence that the SH2 and SH3 domains are essential for the regulation of SFK, and Csk can be recruited to the membrane where SFKs are in an active state. This process is mediated by a Csk-binding protein (Cbp, also called PAG), which binds tightly to the SH2 domain of Csk (5). Activation of SFK by extracellular stimuli leads to the tyrosine phosphorylation of Cbp, generating docking sites for Csk. The recruitment of Csk forms a feedback mechanism for termination of SFK function (6).
- Nada, S. et al. (1991) Nature 351, 69-72.
- Nada, S. et al. (1993) Cell 73, 1125-35.
- Lee, S. et al. (2003) Proc Natl Acad Sci U S A 100, 14707-12.
- Imamoto, A. and Soriano, P. (1993) Cell 73, 1117-24.
- Kawabuchi, M. et al. (2000) Nature 404, 999-1003.
- Matsuoka, H. et al. (2004) J Biol Chem 279, 5975-83.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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