CellSimple^™ Cellular Stress Antibody Assay Kit

Storage

Specificity / Sensitivity

Species Reactivity:

Human, Mouse

Product Description

Background

The CellSimple^™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.
p38 MAP kinase (MAPK) is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors. p38 MAPK is activated by phosphorylation at Thr180 and Tyr182 by various MAPK kinases. Once activated the p38 MAPK phosphorylates a wide spectrum of cellular targets to help the cells adapt and cope with noxious conditions (1). Histone H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks. DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents results in rapid phosphorylation of H2A.X at Ser139. This very early event in the DNA-damage response is required for recruitment of a multitude of DNA-damage response proteins. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals (2).