SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit (#12824) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-P

Product Information

Product Usage Information

1. To prepare the Ultra Blue/AP chromogen-substrate working solution, add 1 drop SignalStain^® Ultra Blue Chromogen Concentrate to 1 mL SignalStain^® Ultra Blue Diluent. Mix well before use. Working solutions are stable for up to 1 day when stored at 4ºC. For optimal results, prepare fresh reagent.

2. After incubation with SignalStain^® Boost IHC Detection Reagent or other AP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 8-20 minutes generally provides an acceptable staining intensity.

3. Wash slides for 5 minutes in water.

4. Counterstain, if desired. Note that Hematoxylin #14166 is unlikely to provide sufficient contrast with Ultra Blue.

5. Mount coverslips. After rinsing off the counterstain, air dry the slides for 20 minutes or longer. Permanently mount coverslips with SignalStain^® Aqueous Mounting Medium #27290. Alternatively, slides may be dehydrated through graded alcohols and cleared in a xylene substitute, then mounted with a permanent mounting medium, SignalStain^® Mounting Medium #14177. Ultra Blue is not compatible with xylene.

For best color preservation and long-term slide storage, it is recommended to use SignalStain^® Mounting Prep Solution #50815 after counterstaining.

Appropriate personal protective equipment should be worn when handling Ultra Blue. Dispose of in accordance with local regulations.

Storage

Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 6 months upon receipt if the reagents are not combined.

Product Description

The SignalStain^® Ultra Blue Alkaline Phosphatase Substrate Kit contains all of the necessary reagents to prepare a working solution of Ultra Blue for staining tissue sections. The Ultra Blue working solution is compatible with alkaline phosphatase (AP) detection systems such as the SignalStain^® Boost IHC Detection Reagents (AP, Rabbit #18653, AP, Mouse #31926, AP, Rat #15764, and AP, Goat #26927), yielding a heat stable, blue reaction product that is also fluorescent.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专